Tuesday, August 23, 2011

Endometrial Biopsy of the Uterus video

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To evaluate the clinical usefulness of examining endometrial biopsy specimens for aromatase cytochrome P-450 as a diagnostic test for endometriosis.
Design: Retrospective, case-controlled study.
Setting: Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
http://photos3.fotosearch.com/bthumb/LIF/LIF139/NU113017.jpg
Patient(s): One hundred five women of reproductive age with normal menstrual cycles underwent endometrial biopsy laparotomy or laparoscopy, and examination of their tissue revealed endometriosis, adenomyosis, and/or leiomyomas. Patients who had cervical carcinoma in situ but no other gynecologic disease were considered to be disease-free.
Intervention(s): Endometrial biopsy specimens were collected.
Main Outcome Measure(s): The expression of aromatase cytochrome P-450 was examined by reverse transcription-polymerase chain reaction and immunohistochemical analysis. The distribution and intensity of the immunostaining was assessed using a semiquantitative index designed H-score.
Result(s): Immunostaining for aromatase cytochrome P-450 was detected in biopsy specimens obtained from patients with endometriosis, adenomyosis, and/or leiomyomas but not in specimens obtained from disease-free patients (H-score <20), with a sensitivity and specificity of 91% and 100%, respectively.
Conclusion(s): The expression of aromatase cytochrome P-450 in biopsy specimens of eutopic endometrium distinguishes between disease-free women and women with endometriosis, adenomyosis, and/or leiomyomas. This technique can be used at outpatient infertility clinics as an initial screening procedure to rule out the presence of estrogen-dependent disease.
Author Keywords: Endometriosis; adenomyosis; leiomyomas; infertility; aromatase cytochrome P-450; estrogen; endometrial biopsy; diagnostic test


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